Question

In the case of ligand binding by the expressed protein, the library can be screened by ______________

(a) immunochemically, by the use of antibodies

(b) ligands if the sequence we are looking for encodes a protein specific to a ligand

(c) using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

(d) all of the mentioned

This question was posed to me during an online exam.

My enquiry is from Screening by Expression In Vivo in division Screening Libraries of Genetic Engineering

Answer 1

Right answer is (d) all of the mentioned

The best I can explain: There are various ways to screen such a library. It can be done immunochemically i.e. by the use of antibodies. Ligands can also be used which bind to the specific proteins encoded by the sequence. There are also cases in which specific DNA sequences bind to the protein encoded by the sequence of interest.