Question

If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

(a) The extract in which the host DNA constructs are incubated is capable of translation only

(b) The extract in which the host DNA constructs are incubated is capable of transcription only

(c) The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

(d) Non radiolabelled amino acids are used

The question was posed to me during an internship interview.

I'd like to ask this question from Expression of Coding Section in-Vitro in division Screening Libraries of Genetic Engineering

Answer 1

Right option is (c) The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

Explanation: If an expression of the coding sequence is to be carried in vitro, then the extract in which host DNA constructs are incubated is capable of both transcription and translation. The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc. Radiolabelled amino acids are used and thus detection becomes easier.