Question

In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

(a) DNA binding, activation

(b) Activation, DNA binding

(c) Activation, transcription

(d) DNA binding, transcription

The question was asked during an interview.

This intriguing question comes from Reporter Genes in section Screening Libraries of Genetic Engineering

Answer 1

Correct choice is (a) DNA binding, activation

To explain I would say: The two hybrid-screening system is based on the fact that many transcriptional activators consist of two domains, the DNA binding domain and activation domain. The activator binds through the DNA binding domain to a sequence upstream of the gene under its control. The activation domain stimulates transcription.