Question

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

(a) It stands for Green Fluorescent Protein

(b) It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light

(c) The blue light is produced because of binding of sodium ions

(d) The absorbed blue light produces green light which can be detected further

I got this question in an international level competition.

This interesting question is from Reporter Genes in chapter Screening Libraries of Genetic Engineering

Answer 1

Correct choice is (c) The blue light is produced because of binding of sodium ions

Easiest explanation: It stands for green fluorescent protein and is obtained from a bio-luminescent jellyfish. The jellyfish produces protein aequorin which produces blue light. This blue light is produced because of binding Calcium ions. The absorbed blue light emits green light which is detected further.