Question

If a host other than E.coli is to be used, what property of DNA to be inserted is disadvantageous?

(a) Circular DNA

(b) Linear DNA

(c) Replicating DNA

(d) Non-replicating DNA

This question was posed to me in unit test.

This interesting question is from Bacteria in portion Using Other Organisms of Genetic Engineering

Answer 1

Correct answer is (c) Replicating DNA

The best explanation: If the DNA is to be inserted in a host other than E.coli than the DNA can be circular or linear and it should be non-replicating. As it is non-replicating, it can be stably maintained in the host only by integration into replicon.