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Natural transformation can be relieved by the use of protoplasts, in the presence of osmotic buffer and polyethylene glycol. What are protoplasts?

(a) Protoplasts are the cells from which cell membrane has been removed

(b) Protoplasts are the cells from which cell wall has been removed

(c) Protoplasts are the cells from which vacuole has been removed

(d) Protoplasts are the cells from which golgi bodies are removed

I have been asked this question in final exam.

The doubt is from Bacteria in chapter Using Other Organisms of Genetic Engineering

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Correct option is (b) Protoplasts are the cells from which cell wall has been removed

Best explanation: Protoplasts are the cells from which the cell wall has been removed. They help in relieving natural transformation in the presence of osmotic buffer and polyethylene glycol.

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