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Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

(a) lithium acetate

(b) calcium phosphate

(c) sodium chloride

(d) aluminum sulphate

I have been asked this question in homework.

My question is from Cultured Cells in Mammals and Restricted Areas in Intact Organisms in division Using Other Organisms of Genetic Engineering

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Right option is (b) calcium phosphate

Explanation: Presence of wild type in HGPRT—in the presence of calcium phosphate led to DNA uptake and stable transformation.

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