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Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

(a) initiating protein synthesis

(b) inhibiting protein synthesis

(c) initiating DNA binding process

(d) inhibiting DNA binding process

I had been asked this question in semester exam.

The above asked question is from Cultured Cells in Mammals and Restricted Areas in Intact Organisms topic in portion Using Other Organisms of Genetic Engineering

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The correct answer is (b) inhibiting protein synthesis

For explanation I would say: Hygromycin is used as a protein synthesis inhibitor. It is conferred by a bacterial hph gene which encodes hygromycin phosphotransferase. Resistance to hygromycin is used as a selectable marker.

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