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ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________

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ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________

(a) gene targeting

(b) knocking out

(c) knocking in

(d) gene disruption

This question was addressed to me in an online quiz.

This intriguing question originated from Generation of Transgenic Organsims topic in division Using Other Organisms of Genetic Engineering

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Correct option is (a) gene targeting

To explain I would say: ES cells are used in order to ensure that insertion is done at the required chromosomal location and the process is termed as gene targeting. In some cases, it is not necessary to ensure that integration is taking place at a normal chromosomal location.

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