Question

The gene targeting approach produces individuals which are ______ for inactivation of the gene.

(a) homozygous

(b) heterozygous

(c) either only homozygous or only heterozygous

(d) both heterozygous and homozygous

This question was posed to me during an interview for a job.

This interesting question is from Generation of Transgenic Organsims in division Using Other Organisms of Genetic Engineering

Answer 1

Correct answer is (b) heterozygous

The best explanation: The gene targeting approach produces individuals which are heterozygous for inactivation of the gene. But it is necessary to generate homozygotes and they are produced by crossing heterozygous individuals and then screening is carried out.