Question

Which of the following is not suitable if the PCR product is non-specific?

(a) Adjusting the concentration of magnesium ions

(b) Increasing annealing temperature

(c) Using touchdown PCR

(d) Using inverse PCR

This question was addressed to me by my college director while I was bunking the class.

This question is from Applications of Genetic Engineering topic in division Genetic Engineering of Genetic Engineering

Answer 1

The correct option is (d) Using inverse PCR

Easy explanation: In case, products are non-specific then sequence can’t be known directly. Optimization of PCR should be carried out and various other strategies should be used such as adjusting the concentration of magnesium ions, increasing annealing temperature or using touch-down PCR.