Question

How can PCR product be cloned into a vector?

(a) It can be done only when PCR products are blunt-ended

(b) It can be done only by restriction enzyme digestion

(c) Both the methods can be used

(d) The blunt-ended approach is favoured

The question was posed to me in a job interview.

The question is from Modifications in PCR in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct answer is (b) It can be done only by restriction enzyme digestion

To explain I would say: PCR product can be cloned into a vector if the DNA molecules are blunt ended or it can also be done by restriction enzyme digestion. In restriction enzyme digestion restriction sites are introduced.