Question

Primers are generally ____________

(a) 20-30 nucleotides long

(b) 40-50 nucleotides long

(c) as long as the template is

(d) taken according to the amount available

I got this question during an interview.

My query is from Primers topic in division Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct choice is (a) 20-30 nucleotides long

For explanation I would say: Primers are generally short in length. They are 20-30 nucleotides long. It is easier to match short primers with the template in comparison to long primers. But in the case of eukaryotic DNA as a template, long primers are preferred.