Question

How many approaches are there for measuring the quantity of PCR products?

(a) 1

(b) 2

(c) 3

(d) 4

The question was asked in semester exam.

My question is taken from Modifications in PCR in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right choice is (b) 2

The explanation: There are basically two approaches for measuring the amount of PCR products. These are known as end-point approach and real time approach. In end-time approach, the amount is measured once the PCR is done. In real time approach the amount is measured while the PCR reaction is still going on.