Question

In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

(a) It allows detection of all double stranded molecules

(b) The reporter and quencher are the molecules present on the same probe

(c) The quencher is having a fluorescent group

(d) Fluorescence is observed only when both the groups are present in proximity to each other

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I need to ask this question from Modifications in PCR in division Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct option is (b) The reporter and quencher are the molecules present on the same probe

The explanation: This method allows the detection of only specific molecules. It is so because the probes are specific for areas to be amplified. The probe consists of reporter at one end and quencher at the other. The reporter consists of the fluorescent group which fluoresce only when the quencher is released. Quencher is released as a specific binding of the probe is taking place.