Question

Which of the statement holds for long-range PCR and in its relation?

(a) It is the PCR in which longer templates are used

(b) DNA polymerases which don’t have proof-reading activity give larger products

(c) DNA polymerases’ processivity is not a measure to have larger products

(d) It is PCR in which a mixture of enzymes is used to have larger products

I have been asked this question in an interview for internship.

The origin of the question is Precautions and Drawbacks topic in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct option is (d) It is PCR in which a mixture of enzymes is used to have larger products

Explanation: Long range PCR is the PCR in which a mixture of enzymes is used to have larger products. If DNA polymerases are having proof reading activity then we can obtain larger products because in this case chain terminators are not used. Also, the processivity of the enzymes is also very important.