Question

Which of the following is not a condition for PCR?

(a) Initial melting carried out for 5 minutes at 94 degrees

(b) Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees

(c) Renaturation for 5 minutes at 60 degrees

(d) DNA synthesis at 72 degrees for 1.5 minutes

The question was posed to me during an online exam.

The above asked question is from The Basic Technique in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct choice is (c) Renaturation for 5 minutes at 60 degrees

For explanation: The melting temperature is 94 degrees and the initial melting is carried for 5 minutes at this temperature. It is followed by 30 cycles each consisting of melting for 1 minute at 94 degrees. The renaturation is carried for 1 minute at 60 degrees. The DNA synthesis is carried out at 72 degrees for 1.5 minutes. After the 30 cycles, a final round of extension is carried out for 10 minutes.