Question

Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.

(a) True

(b) False

The question was posed to me by my college director while I was bunking the class.

This key question is from The Basic Technique topic in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct answer is (b) False

The best I can explain: Primers and polymerases are not added more as the reaction proceeds. It is so because the polymerase is heat stable and is not destroyed during the reaction. Primers on the other hand are added in excess at the beginning of the reaction.