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In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

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in Genetic Engineering by (118k points)
In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

(a) Renaturing

(b) Denaturing

(c) The routine agarose gel

(d) The routine polyacrylamide gel

This question was posed to me in homework.

Origin of the question is Gel electrophoresis, Oligonucleotide  & Microarrays in division Basics of Genetic Engineering of Genetic Engineering

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Correct option is (b) Denaturing

Explanation: In the case of single stranded molecules, denaturing gels are used. It is so because, if denaturing gels are not there, there are chances of formation of secondary structures which hinder the movement of nucleic acids. Thus to avoid this, denaturing agents such as urea, etc. are added.

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