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The greatest separation is obtained in which portion of the gel?

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in Genetic Engineering by (118k points)
The greatest separation is obtained in which portion of the gel?

(a) Lower portion where the anode is

(b) Lower portion where the cathode is

(c) The separation is uniform all over

(d) It varies according to the quantity of the size of the molecules to be separated

I have been asked this question at a job interview.

This intriguing question originated from Gel Electrophoresis, Oligonucleotide  & Microarrays topic in section Basics of Genetic Engineering of Genetic Engineering

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Correct choice is (a) Lower portion where the anode is

The explanation: The greatest separation is obtained in the lower portion of the gel where the anode is. Thus the smaller molecules are having the greatest separation because they are the one which travels farther in the gel.

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