Question

A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?

(a) Filtration and centrifugation

(b) Filtration or centrifugation

(c) Allowing to sediment

(d) By passing through a silica column

I got this question during an internship interview.

The query is from Gel Electrophoresis, Oligonucleotide  & Microarrays topic in chapter Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct choice is (b) Filtration or centrifugation

To explain: As the target DNA is crushed and soaked into the buffer, the majority of it diffuses into the gel. It can be separated via filtration or separation. It is simply termed as recovering the DNA from the gel by diffusion.