Question

Which of the statements is correct for non uniform separation obtained other than the conventional one?

(a) It can be obtained via applying strong field towards the cathode

(b) It can be obtained via applying the same field on both the sides

(c) The separation is greater on the side of stronger field

(d) Its is greater towards the weaker field

I have been asked this question in quiz.

The origin of the question is Gel Electrophoresis, Oligonucleotide  & Microarrays in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct choice is (c) The separation is greater on the side of stronger field

Best explanation: Conventionally, separation is greater towards the lower portion i.e. where the anode is. In order to obtain greater separation towards the cathode, the stronger field is applied here. As the field is weaker on the anode side, the molecules retard as they move towards anode and are less separated.