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Which of the following components settles at the bottom?

(a) RNA

(b) Proteins

(c) Nicked DNA

(d) Supercoiled DNA

I got this question by my college professor while I was bunking the class.

This question is from Purification of Plasmid DNA topic in portion Basics of Genetic Engineering of Genetic Engineering

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Best answer
The correct choice is (a) RNA

Explanation: The component settling at the bottom is RNA. And the proteins float on the free surface. The nicked DNA forms a band above the supercoiled form.

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