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in Genetic Engineering by (118k points)
The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol.

(a) False

(b) True

The question was asked in exam.

Enquiry is from Purification of Plasmid DNA topic in chapter Basics of Genetic Engineering of Genetic Engineering

1 Answer

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Correct answer is (b) True

To elaborate: The nucleic acid is present in the solution and is precipitated by the addition of sodium or ammonium acetate and ethanol. It is because; nucleic acid is polar in nature and thus easily dissolves in water. Hence, to avoid this sodium acetate and ethanol is added. Sodium acetate is shields the charge present on the sugar phosphate backbone and further bonds are easily formed between ethanol and phosphate. It leads to separating out of nucleic acids.

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