Question

Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?

(a) Cell lysis

(b) Removal of proteins

(c) Removal of chromosomal DNA

(d) Dissolving plasmid in water

The question was posed to me during a job interview.

Enquiry is from Purification of Plasmid DNA topic in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

Right option is (d) Dissolving plasmid in water

Best explanation: There are some basic steps which are included in obtaining plasmid DNA from E. coli. Firstly, the cell is lysed, further removal of proteins and chromosomal DNA is done. A plasmid is obtained and collected but not in water. Also, further purification is done if necessary.