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E. coli DNA ligase doesn’t require a cofactor.

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in Genetic Engineering by (118k points)
E. coli DNA ligase doesn’t require a cofactor.

(a) True

(b) False

I have been asked this question during an online exam.

I want to ask this question from Enzymes for Ligation in chapter Basics of Genetic Engineering of Genetic Engineering

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The correct answer is (b) False

The best I can explain: The E. coli DNA ligase requires a cofactor nicotamine adenine dinucleotide (NAD), along with 5’ phosphate group and 3’ hydroxyl group.

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