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If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

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in Genetic Engineering by (118k points)
If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

(a) E. coli DNA ligase

(b) T4 DNA ligase

(c) Both of these enzymes act equally in carrying out blunt ended ligations

(d) None of them is able to carry out blunt ended ligations

The question was asked in examination.

My question is from Enzymes for Ligation in section Basics of Genetic Engineering of Genetic Engineering

1 Answer

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Best answer
Right answer is (b) T4 DNA ligase

Easy explanation: If blunt ended ligations are to be carried out, T4 DNA ligase should be used. E. coli DNA ligase is unable to carry out blunt ended ligation.

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