Question

If the DNA samples are to be analyzed, the membrane should be studied. Which of them is correct for it?

(a) The membrane should not be treated with UV or heat

(b) The membrane is further immersed in the buffer containing labelled nucleic acids which are known  as strips

(c) Labelled nucleic acids bind to the sequences which are same as the sequences present on the membrane

(d) The binded sequences can be visualized by autoradiography

This question was addressed to me during an interview.

The question is from Gel electrophoresis, Oligonucleotide  & Microarrays topic in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct option is (d) The binded sequences can be visualized by autoradiography

The explanation: The membrane should be treated with UV or heated. It ensures the cross linking of the DNA molecules on the membrane. The labelled nuclei in the buffer are called a probe. These sequences bind to the complementary sequences on the membrane and can be visualized via autoradiography.