Question

If the amount of agarose added is more, the molecular under analysis should have the following characteristic:

(a) small size

(b) large size

(c) size has no relation with the amount of agarose

(d) the amount of molecules under analysis matters

I have been asked this question in class test.

Question is from Gel electrophoresis, Oligonucleotide  & Microarrays topic in chapter Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct option is (a) small size

To elaborate: In the case of agrose gels, the separation takes place because of size. If the amount of agarose is more, smaller size molecules will be able to move easily as in comparison to larger size molecules. It is so because; the movement is through the pores. More the amount of agarose, smaller the size of the pore.