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If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

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in Genetic Engineering by (118k points)
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

(a) Wedge gels

(b) Buffer-gradient gels

(c) Pulse field gels

(d) Varying the amount of agarose will carry out the separation

The question was posed to me in exam.

My question is taken from Gel Electrophoresis, Oligonucleotide  & Microarrays topic in division Basics of Genetic Engineering of Genetic Engineering

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Correct option is (c) Pulse field gels

Explanation: In the case, if the molecules to be separated are very large, say the chromosomes are to be separated. In this case, pulse-field gels are used. It is termed as pulse field gel electrophoresis (PFGE).

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