Question

Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

(a) They are obtained via polymerization between acrylamide and bis-acrylamide

(b) The components added for initiating polymerization are ammonium persulphate and TEMED

(c) It is casted in horizontal and flat trays

(d) TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

This question was posed to me by my school teacher while I was bunking the class.

Asked question is from Gel electrophoresis, Oligonucleotide  & Microarrays topic in chapter Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct option is (c) It is casted in horizontal and flat trays

To explain I would say: Polyacrylamide gels are made up by the polymerization of acryl amide and bis acryl amide units. The polymerization takes place because of TEMED and ammonium persulphate, as TEMED catalyses the formation of free radicals from persulphate. These gels are usually casted in vertical trays whereas the agarose gels are casted in horizontal and flat trays.