Question

Another method of DNA recovery is termed as freeze-squeeze. The correct statement for it is?

(a) The slice containing DNA is cut and frozen into liquid oxygen

(b) Freezing doesn’t have any effect on the DNA structure

(c) After freezing centrifugation is carried out through glass wool plug

(d) The substance used for centrifugation allows the gel to pass through but the liquid is retained

I had been asked this question in unit test.

The origin of the question is Gel Electrophoresis, Oligonucleotide  & Microarrays topic in portion Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct answer is (c) After freezing centrifugation is carried out through glass wool plug

Explanation: It is also a method for obtaining DNA from gels. The slice containing DNA is cut and frozen into liquid nitrogen. Freezing in liquid nitrogen leads to disruption of structure. As the centrifugation by glass wool plug is carried out after freezing out, the gel is retained by it. The liquid which is containing dissolved DNA will be allowed to pass through.