Question

Which of the following steps is not included in the transferring of DNA?

(a) The gel containing the DNA is immersed in the buffer in the reservoir

(b) The buffer flows up through the gel via a wick

(c) Membrane is placed over the gel

(d) The nucleic acids are dissolved in the buffer and are obtained from it via pipetting

I have been asked this question during an internship interview.

This intriguing question originated from Gel electrophoresis, Oligonucleotide  & Microarrays in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct option is (d) The nucleic acids are dissolved in the buffer and are obtained from it via pipetting

Easy explanation: The gel containing the DNA molecules is immersed in the buffer. The membrane is placed over the gel. The buffer rises from the wick and passes through the gel. It carries along with it, the nucleic acids and they are then trapped by the membrane. The membrane can be washed further in order to obtain the DNA.