Question

Melting temperature is given by ____________

(a) 4(G+C) + 2(A+T)

(b) 2(G+C) + 4(A+T)

(c) 2(A+G) + 4(C+T)

(d) 4(A+G) + 2(C+T)

I have been asked this question during a job interview.

The query is from Primers topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct answer is (a) 4(G+C) + 2(A+T)

The explanation is: The melting temperature is that temperature at which the primers associate with the template. The melting temperature is decided by the amount of different nucleotides present.