Question

Which end of the primer should be matched properly in order to carry out the amplification?

(a) 5’ end

(b) 3’ end

(c) Both of the ends should be matched properly

(d) Anyone of the ends should match

This question was posed to me by my college director while I was bunking the class.

I need to ask this question from Primers topic in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct answer is (b) 3’ end

To elaborate: For carrying out the amplification, it is not necessary that the whole primer should match with the template. But it is necessary that 3’ end should match because if 3’ end is not matched the polymerase won’t be able to carry out elongation.