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Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

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Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

(a) Amination of bases

(b) Chemical cross-linking between the strands

(c) Chemical cross-linking within the strands

(d) Slowing down the polymerase

I got this question in an interview for internship.

The query is from Precautions and Drawbacks in chapter Polymerase Chain Reaction of Genetic Engineering

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The correct answer is (a) Amination of bases

To explain: There are various reasons for DNA damage such as chemical cross-linking both between and within the strands. Deamination of bases is also one of the reasons. If polymerase is slowed down there are chances of incorporation of incorrect bases.

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