Question

Which can be used as a precaution in order to minimize contamination?

(a) Careful use and design of pipettes

(b) Placing the pre-PCR and post-PCR stages in the same rooms

(c) Extracting the DNA along with surface layers

(d) Use of primers carefully is not very important

I have been asked this question in class test.

The origin of the question is Precautions and Drawbacks topic in division Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right choice is (a) Careful use and design of pipettes

Explanation: Pipettes are very important in minimizing contamination. Thus they should be designed and used carefully. The pre-PCR and post-PCR stages should be places in separate rooms. While extraction of DNA surface layers should be removed because they might be containing bacteria. Sometimes the use of species specific primers is also very important, thus they should be used carefully.