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How can the specificity of primer annealing be increased?

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in Genetic Engineering by (118k points)
How can the specificity of primer annealing be increased?

(a) Use of short primers

(b) Raising temperature

(c) Adjusting the concentration of sodium ions

(d) Using polymerase with proof reading activity

I got this question in a national level competition.

The doubt is from Precautions and Drawbacks in section Polymerase Chain Reaction of Genetic Engineering

1 Answer

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Best answer
Right option is (b) Raising temperature

Explanation: The specificity of primers annealing can be increased in various ways such as increasing temperature, using long primers. If long primers are used there are increased chances of having correct matching. Also, if the magnesium ion concentration is adjusted, annealing can be done more effectively. It is so because they stabilize primer-template binding.

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