Question

The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.

(a) True

(b) False

This question was posed to me in quiz.

The query is from Modifications in PCR topic in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right answer is (a) True

For explanation I would say: The primer annealing temperature is often very low from the maximum temperature. Though the low temperature is for stable binding of the template and the primer but at times it leads to mismatches.