Question

Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?

(a) It is possible to incorporate the reagents in a lipid drop

(b) It refers to the PCR carried out at large scale

(c) The temperature variation is not possible easily

(d) If there is a single template molecule at the start then amplification results in the mixture and it because of the extent of extension carried out

I got this question by my college director while I was bunking the class.

Query is from Modifications in PCR in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct choice is (a) It is possible to incorporate the reagents in a lipid drop

To explain: It is possible to incorporate the reagents in a lipid drop. In this type, PCR is carried out at a small scale. The temperature of these drops can be varied easily. If there is a single template in the DNA molecule then the amplification results in only one type of molecules.