Question

Which type of PCR allows the use of permeabilized tissue?

(a) Inverse PCR

(b) In-situ PCR

(c) Quantitative PCR

(d) Hot-start PCR

The question was posed to me in semester exam.

The doubt is from Modifications in PCR topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right choice is (b) In-situ PCR

Easiest explanation: In-situ PCR allows the use of permeabilized tissue, such as thin sections on a microscopic slide. The PCR product is detected by hybridization and it allows locating the nucleic acid in the target tissue.