Question

Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

(a) The basis is that extension is not started until the first cycle reaches its maximum temperature

(b) The polymerase is added after the first cycle has reached its maximum temperature or melting temperature

(c) It is satisfactory for small number of samples

(d) It leads to generation of non specific products

I had been asked this question in unit test.

Query is from Modifications in PCR in division Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct option is (d) It leads to generation of non specific products

Easy explanation: Hot-start PCR is a modification which is used in order to overcome the problem of the extension before the maximum temperature of first cycle is reached. Hence, polymerase is added only after the maximum temperature for first cycle is reached. Hence, there are more specific products which are generated because proper annealing of primers has taken place. It is suitable for a small number of samples but not for a large number of samples.