Question

Which of the statement hold true for Quantitative PCR?

(a) A fluorescent dye is used which binds on single stranded DNA molecules

(b) SYBR green is an example such type of dye

(c) The quantity of DNA is simply measured by measuring the amount of fluorescence

(d) This approach is useful if the products are non-specific in nature

This question was posed to me in examination.

Asked question is from Modifications in PCR topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right answer is (c) The quantity of DNA is simply measured by measuring the amount of fluorescence

Best explanation: A fluorescent dye is used which binds on double stranded DNA molecules and SYBR green is an example of such type of dye. The quantity of DNA is measured by simply measuring the amount of fluorescence. It is used only in the case if products are created specifically because it simply relies on binding of the dye to double stranded molecules.