Question

Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it?

(a) It is used for increasing copy number in plasmids containing pMB1 origin

(b) Chloramphenicol promotes bacterial protein synthesis

(c) The protein synthesis is responsible for chromosomal DNA replication

(d) Chromosomal DNA replication is related to cell division

The question was posed to me in an interview for job.

Enquiry is from Vectors topic in portion Simple Cloning of Genetic Engineering

Answer 1

Correct answer is (b) Chloramphenicol promotes bacterial protein synthesis

The explanation: Chloramphenicol amplification is used for increasing the copy number in plasmids containing the pMB1 origin. It is responsible for inhibiting bacterial protein synthesis. As the bacterial protein synthesis is inhibited, chromosomal DNA replication is also inhibited. Chromosomal DNA replication is related to cell division and it is also blocked. But the plasmid replication is not inhibited and it replicates.