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Which of the statement is true for pBR322?

(a) It contains only an ampicillin resistance gene

(b) It contains both ampicillin resistant and tetracycline resistant gene

(c) The cloning site is present only in the ampicillin resistant gene

(d) It is a natural vector

The question was posed to me by my school teacher while I was bunking the class.

My doubt stems from Vectors topic in portion Simple Cloning of Genetic Engineering

1 Answer

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Best answer
Correct choice is (b) It contains both ampicillin resistant and tetracycline resistant gene

Explanation: pBR 322 is the man-made vector. It contains both ampicillin resistant and tetracycline resistant genes. The cloning site is also present in both of the genes.

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