Question

Linkers are often used in cloning. Choose the incorrect statement for linkers.

(a) These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence

(b) They are blunt ended molecules

(c) They are ligated to staggered ended insert molecules by T4 DNA ligase

(d) After treatment with enzyme, both the ends of the linker are staggered

The question was posed to me in an online quiz.

I need to ask this question from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes topic in chapter Simple Cloning of Genetic Engineering

Answer 1

The correct option is (c) They are ligated to staggered ended insert molecules by T4 DNA ligase

To elaborate: Linkers are often used in cloning. These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence. They are blunt ended molecules and are ligated to the blunt ended insert molecules by T4 DNA ligase. After treatment with the enzyme, both the ends of the linker are staggered.