Question

There is no effect if the insert itself contains the restriction site.

(a) True

(b) False

I had been asked this question in class test.

This intriguing question comes from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes in section Simple Cloning of Genetic Engineering

Answer 1

Correct choice is (b) False

The best I can explain: If the insert itself is having a restriction site, the insert itself is cleaved when the restriction enzyme is cleaved along with the linker. To avoid this, the methylation of the site in the insert is done.