Question

The transfer of plasmid from one bacterial cell to another when cargo and conjugal plasmids are used, it is usually is carried out by _____ mating.

(a) diparental

(b) uniparental

(c) triparental

(d) multiparental

I had been asked this question in an interview for job.

My question is taken from Bacteria topic in chapter Using Other Organisms of Genetic Engineering

Answer 1

Right answer is (c) triparental

To explain: The transfer is carried out by triparental mating. Three conjugants are used, E.coli carrying the conjugal plasmid, E.coli containing cargo and helper plasmid and the recipient bacterial species to be manipulated.