Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
A wide range of host cell lines are available and...

A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

← Prev Question Next Question →

+1 vote

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

(a) kidney

(b) liver

(c) lymphoblast from leukaemia patient

(d) both kidney and lymphoblast from a leukaemia patient

I have been asked this question in an online interview.

This interesting question is from Cultured Cells in Mammals and Restricted Areas in Intact Organisms topic in portion Using Other Organisms of Genetic Engineering

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

The correct choice is (d) both kidney and lymphoblast from a leukaemia patient

To elaborate: There is a wide range of host cell lines available. Commonly used human cell lines are HeLa 293T, obtained from kidney and Jurkat which is obtained from lymphoblast from a leukaemia patient.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Constitutive promoters are majorly obtained from ________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

In ___________ cells, virus replication doesn’t take place and viral DNA ___________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

SV40 is a virus and it produces how many transcripts?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

The transfer of plasmid from one bacterial cell to another when cargo and conjugal plasmids are used, it is usually is carried out by _____ mating.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
bacteria

+1 vote

1 answer

T protein is required for replication. It is a product of ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

If plasmids direct their own transfer from one bacterium cell to another, then they are called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
bacteria

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (481)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (484)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...