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Cells deficient in thymidine kinase (TK) are also...

Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

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+1 vote

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

(a) True

(b) False

The question was posed to me in my homework.

This intriguing question originated from Cultured Cells in Mammals and Restricted Areas in Intact Organisms in section Using Other Organisms of Genetic Engineering

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

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1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

The correct option is (a) True

To explain I would say: Cells deficient in thymidine kinase are also killed in HAT medium. It is so because pyrimidine synthesis is also blocked in the presence of aminopterin and utilization of thymidine in the HAT medium requires a functional TK.

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